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Industrial Oil Products

Session:: General Industrial Oil Products

Fermentation parameters for improving 2,3-butanediol output while limiting acetoin generation using lignocellulosic hydrolysates

Tuesday, May 2, 2023
4:10 PM – 4:30 PM MDT
Room: 403
Abstract: The development of a bioeconomy aims to alleviate two problems: (1) improve the economic output of rural areas and (2) establish an industry generating bio-based fuels or chemicals to reduce the utilization of petroleum-based products. This can be accomplished by implementing biochemical conversion processes using sugars from plant biomass to generate platform chemicals that can be upgraded to higher value fuels or chemicals. One platform chemical is 2,3-butanediol (2,3-BDO) that can serve a wide array of applications, but it is most attractive for chemical upgrading to sustainable aviation fuels (SAF). In this work corn stover and switchgrass were subjected to a “green liquor” pretreatment that utilized sodium carbonate produced from captured and absorbed carbon dioxide off-gas from a fermentation process. This form of alkaline pretreatment can preserve most of polysaccharide content in the plant cell wall while providing a substantial amount of delignification. Fermentable sugars from the pretreated corn stover and switchgrass were recovered following enzymatic hydrolysis and then subjected to fermentation using the native producing 2,3-BDO strain Paenibacillus polymyxa (P. polymyxa). Fermentation control parameters (such as dissolved oxygen and pH) were adjusted during fermentation to influence P. polymyxa to generate higher titers of 2,3-BDO instead of the byproduct acetoin. Separation and recovery of 2,3-BDO from fermentation broth was accomplished by way of liquid-liquid extraction. Sufficient product recovery yields were achieved only when high enough concentrations of 2,3-BDO were exhibited in the fermentation broth.