Abstract: The enzymatic hydrolysis of cellulose and the succinylated saccharides from the hydrolysis as the novel emulsifier for emulsion systems was investigated. Two commercial enzymes UltimaseBWL 40 (endoglucanase (EG)/xylanase enzyme mixture) and Celluclast 1.5 L (cellobiohydrolases (CBHs)/ EG mixture) were utilized in this study, and produced cellodextrins by varying enzymatic hydrolysis times were monitored by Reducing sugar, Saccharide Composition, ESI-TOF-MS. With time increasing, the reducing sugar increased for both Ultimase®BWL 40 (2mg/ml-40mg/ml and Celluclast® 1.5 L hydrolysis (4mg/ml-27mg/ml). After enzymatic hydrolysis by both Ultimase®BWL 40 and Celluclast® 1.5 L hydrolysis, saccharides with monomer, dimmer, trimmer, tetramer, and cellodextrins with 3-18 glucose units were generated. Due to the different types of enzymes, saccharides’ composition changed differently with varying hydrolysis times. Saccharides with more than trimmer among saccharides mixture hydrolysed by Ultimase®BWL 40 increased significantly with time increasing, and the one hydrolysed by Celluclast® 1.5 L have not much change. But saccharides with less than or including a trimmer among saccharides mixture hydrolysed by Celluclast® 1.5 L increased significantly with time increasing, and the one hydrolysed by Ultimase®BWL 40 had no significant changes. A higher proportion of saccharides with more than a trimmer was produced after 7h enzymatic hydrolysis of Celluclast® 1.5 L (around 22%) and 24h of Ultimase®BWL 40 (around 32%). The succinylated products verified by Fourier Transform Infrared (FTIR), exhibited a lower contact angle (around 50 degrees) on the non-polar surface with excellent emulsifying ability, which could stabilize the fish oil-in-water emulsion containing up to 70wt% fish oil content with 536.57±9.33 nm droplet size for SC7-SAC12, 507.73±5.01nm for SB24-SAC12. Overall, this work gives a guideline to hydrolysis cellulose into cellodextrins in the green approach and the fraction of novel emulsifiers with high concentrated emulsion system.
