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Biotechnology

Gene Editing Technologies

Tuesday, May 2, 2023
8:25 AM – 10:30 AM MDT
Room:402

This session will explore how gene editing can enhance grain and pulse crops. Pea is limited in the food industry by a residual flavor in pulse flour. CRISPR/Cas9-mediated gene editing technology was used to reduce the enzyme that leads to negative taste and smell while increasing linoleic acid content. Also, genetic engineering tools modified aspects of sorghum that drive gene expression for water usage, posing a promising future for engineering complex traits. Finally, by silencing the AiP2 gene, soybean seed protein content was increased, with no significant decrease in seed oil content.

Presentations:

  • 8:30 AM – 8:50 AM MDT
    CRISPR-mediated precise targeted insertion and its applications in plants

    Presenting Author: Feng Zhang, PhD (he/him/his) – University of Minnesota

    Co-Author: Jitesh Kumar – University of Minnesota

  • 8:50 AM – 9:10 AM MDT
    Development of synthetic genetic circuits to improve water-use efficiency in sorghum

    Presenting Author: Janina Tamborski (she/her/hers) – Stanford University

    Co-Author: Jennifer A N Brophy – Stanford University

  • 9:10 AM – 9:30 AM MDT
    CRISPR-Cas mediated genetic manipulation of lipoxygenase, precursor fatty acids and flavour components in yellow pea

    Presenting Author: Pankaj K. Bhowmik, PhD (he/him/his) – National Research Council Canada

    Co-Author: Brittany Polley (she/her/hers) – National Research Council of Canada

    Co-Author: Wei Yan (she/her/hers) – National Research Council of Canada

    Co-Author: Tom Warkentin (he/him/his) – University of Saskatchewan

    Co-Author: Frederic Marsolais (he/him/his) – Agriculture Agri-Food Canada

    Co-Author: Mehmet C. Tulbek, PhD (he/him/his) – Saskatchewan Food Industry Development Centre

    Co-Author: Mike Nickerson, PhD (he/him/his) – University of Saskatchewan

  • 9:30 AM – 9:50 AM MDT
    DNA-free editing of two genes to optimize cuticular wax structure in Brassica napus

    Presenting Author: Rui Guan, PhD – University of Nebraska-Lincoln/SLU

    Co-Author: Xueyuan Li – Swedish University of Agricutural Sciences

    Co-Author: Lihua Zhu – Swedish University of Agricultural Sciences

    Co-Author: Eu Sheng wang – Swedish University of Agricultural Sciences

    Co-Author: Frederic Baudouin, PhD – Rothamsted Research, UK

  • 9:50 AM – 10:10 AM MDT
    RNAi and CRISPR-Cas silencing E3-ring ubiquitin ligase AiP2 mitigates proteome rebalancing and enhances soybean seed protein content.

    Presenting Author: Eliot M. Herman – University of Arizona

    Co-Author: Bo Shen – Corteva Agrisciences

    Co-Author: Monica Schmidt – University of Arizona

    Co-Author: Anthony J. Kinney, DPhil (he/him/his) – Corteva Agriscience

  • 10:10 AM – 10:30 AM MDT
    Panel discussion